technique lipofilling fat grafting adipsculpt lipomodeling fat transfer

The lipofilling technique is composed of 4 steps:

Infiltration of donor area

Harvesting of adipose tissue

Purification of the harvested tissue

Reinjection of the purified adipose tissue

Each step is essential to ensure high fat retention rates (high graft uptake). To obtain tissue of optimal quality and to improve tissue viability, these key points must be respected.

Since 2008, ADIP’Sculpt have studied lipofilling in the search of the optimal protocol to transfer viable tissue, resulting in several international journal publications.

Infiltration


Infiltration of the donor site is performed to allow the adipose tissue to dissociate prior to harvesting. Furthermore, the presence of adrenalin in the infiltration solution reduces bleeding during liposuction.

However, ADIP’Sculpt research on adipose stem cells highlighted the negative effects of lidocaine on stem cells viability. Therefore, use of local anaesthetic must be avoided when possible.

Lidocaine toxicity on adipose stem cells adipsculpt lipofilling infiltration
adipsculpt cannula great efficiency tissu survival micro lobules lipofilling harvesting

Harvesting / Liposuction


Harvesting of adipose tissue can be performed manually, or with a powered suction device. In either case, care must be taken to control the negative pressure applied to the tissue in order to preserve the adipocytes viability. To ensure this, ADIP’Sculpt developed the SPINFILL syringe to control the negative pressure.

ADIP’Sculpt developed as well specific micro-cannulas, designed to harvest small adipose lobules while preserving the adipocytes viability. The size of the lobules will determine the viability of the graft.

The Purification


Following liposuction, the harvested tissue is mixed with the infiltration solution and cell debris. To improve lipofilling results and to limit graft resorption, the tissue must be concentrated and fluids removed. This can be achieved by decantation, centrifugation or filtration. Centrifugation is the most efficient technique, however, the parameters must be controlled to concentrate the tissue while preserving viability.

ADIP’Sculpt protocol uses gentle centrifugations combined with washing steps to eliminate the negative factors (blood, inflammatory and death factors etc.) present after liposuction.

high graft intake adipsculpt fat grafting lipofilling purification
centrifugation adipocyte death adipsculpt lipofilling graft survival purification

Our washing method present the best results for fat survival

%

Decantation

%

Single centrifugation

%

ADIP'Sculpt protocol : 3 centrifugations/washes

fat re-injection lipofilling adipsculpt high graft intake reinjection

Reinjection


Re-injection is the last step of lipofilling and is of the utmost importance to ensure the greatest graft uptake.

Small lobules of adipose tissue are injected across several planes of the recipient site.
Adipose tissue should be reinjected within 2mm of an existing blood vessel to avoid ischemia of the tissue, and to improve its survival.

Proven clinical results


ADIP’Sculpt products are used by surgeons worldwide. Clinical studies have been performed to consolidate our knowledge in the field of adipose tissue grafting.

MACROFILL protocol is used in combination with DIEP flap to reconstruct women breasts after cancer.

A study demonstrated that one session of lipofilling led to 70% (obtained with 3D quantification) of graft uptake after 4 months , including in irradiated hypotrophic areas.

International Scientific Publications


Effect of Washes and Centrifugation on the Efficacy of Lipofilling With or Without Local Anesthetic.

Girard AC, Mirbeau S, Gence L, Hivernaud V, Delarue P, Hulard O, Festy F, Roche R

PubMed

Autologous Fat Grafting in the Breast: Critical Points and Technique Improvements.

Hivernaud V, Lefourn B, Guicheux J, Weiss P, Festy F, Girard AC, Roche R

PubMed

Effect of centrifugation and washing on adipose graft viability: a new method to improve graft efficiency.

Hoareau L, Bencharif K, Girard AC, Gence L, Delarue P, Hulard O, Festy F, Roche R

PubMed

New insights into lidocaine and adrenaline effects on human adipose stem cells.

Girard AC, Atlan M, Bencharif K, Gunasekaran MK, Delarue P, Hulard O, Lefebvre-d’Hellencourt C, Roche R, Hoareau L, Festy F

PubMed

Autologous fat grafting: A comparative study of four current commercial protocols.

Hivernaud V, Lefourn B, Robard M, Guicheux J, Weiss P

PubMed

Clinical results with MACROFILL.

Nelissen X, Lhoest F, Preud’Homme L

PubMed

The lipofilling technique is composed of 4 steps:

Infiltration of donor area

Harvesting of adipose tissue

Purification of the harvested tissue

Reinjection of the purified adipose tissue

technique lipofilling fat grafting adipsculpt lipomodeling fat transfer

Each step is essential to ensure high fat retention rates (high graft uptake). To obtain tissue of optimal quality and to improve tissue viability, these key points must be respected.

Since 2008, ADIP’Sculpt have studied lipofilling in the search of the optimal protocol to transfer viable tissue, resulting in several international journal publications.

Infiltration


Infiltration of the donor site is performed to allow the adipose tissue to dissociate prior to harvesting. Furthermore, the presence of adrenalin in the infiltration solution reduces bleeding during liposuction.

However, ADIP’Sculpt research on adipose stem cells highlighted the negative effects of lidocaine on stem cells viability. Therefore, use of local anaesthetic must be avoided when possible.

Lidocaine toxicity on adipose stem cells adipsculpt lipofilling infiltration

Harvesting / Liposusction


Harvesting of adipose tissue can be performed manually, or with a powered suction device. In either case, care must be taken to control the negative pressure applied to the tissue in order to preserve the adipocytes viability. To ensure this, ADIP’Sculpt developed the SPINFILL syringe to control the negative pressure.

ADIP’Sculpt developed as well specific micro-cannulas, designed to harvest small adipose lobules while preserving the adipocytes viability. The size of the lobules will determine the viability of the graft.

adipsculpt cannula great efficiency tissu survival micro lobules lipofilling harvesting

The Purification


Following liposuction, the harvested tissue is mixed with the infiltration solution and cell debris. To improve lipofilling results and to limit graft resorption, the tissue must be concentrated and fluids removed. This can be achieved by decantation, centrifugation or filtration. Centrifugation is the most efficient technique, however, the parameters must be controlled to concentrate the tissue while preserving viability.

ADIP’Sculpt protocol uses gentle centrifugations combined with washing steps to eliminate the negative factors (blood, inflammatory and death factors etc.) present after liposuction.

high graft intake adipsculpt fat grafting lipofilling purification
centrifugation adipocyte death adipsculpt lipofilling graft survival purification

Our washing method present the best results for fat survival

%

Decantation

%

Single centrifugation

%

ADIP'Sculpt protocol : 3 centrifugations/washings

Reinjection


fat re-injection lipofilling adipsculpt high graft intake reinjection

Re-injection is the last step of lipofilling and is of the utmost importance to ensure the greatest graft uptake.

Small lobules of adipose tissue are injected across several planes of the recipient site.
Adipose tissue should be reinjected within 2mm of an existing blood vessel to avoid ischemia of the tissue, and to improve its survival.

Proven clinical results


ADIP’Sculpt products are used by surgeons worldwide. Clinical studies have been performed to consolidate our knowledge in the field of adipose tissue grafting.

MACROFILL protocol is used in combination with DIEP flap to reconstruct women breasts after cancer.

A study demonstrated that one session of lipofilling led to 70% (obtained with 3D quantification) of graft uptake after 4 months , including in irradiated hypotrophic areas.

International Scientific Publications


Effect of Washes and Centrifugation on the Efficacy of Lipofilling With or Without Local Anesthetic.

Girard AC, Mirbeau S, Gence L, Hivernaud V, Delarue P, Hulard O, Festy F, Roche R

PubMed

Autologous Fat Grafting in the Breast: Critical Points and Technique Improvements.

Hivernaud V, Lefourn B, Guicheux J, Weiss P, Festy F, Girard AC, Roche R

PubMed

Effect of centrifugation and washing on adipose graft viability: a new method to improve graft efficiency.

Hoareau L, Bencharif K, Girard AC, Gence L, Delarue P, Hulard O, Festy F, Roche R

PubMed

New insights into lidocaine and adrenaline effects on human adipose stem cells.

Girard AC, Atlan M, Bencharif K, Gunasekaran MK, Delarue P, Hulard O, Lefebvre-d’Hellencourt C, Roche R, Hoareau L, Festy F

PubMed

Autologous fat grafting: A comparative study of four current commercial protocols.

Hivernaud V, Lefourn B, Robard M, Guicheux J, Weiss P

PubMed

Clinical results with MACROFILL.

Nelissen X, Lhoest F, Preud’Homme L

PubMed