Protocol & Research
- Tissue harvesting
- Washing and centrifugation
Tissue preservation is key to its survival. Adip’sculpt instruments, technique and protocols ensure efficient tissue harvesting, while controlling the negative pressure and preserving adipose / Stromal Vascular Fraction (SVF) cells, leading to:
- Increased retention rates: 70 to 90%
- Decreased oil release, cyst formation, necrosis, inflammation.
Infiltration of the tumescent solution
Pre-tunneling and infiltration of the tumescent solution (avoid local anaesthetic if the patient is under general anaesthesia as it is toxic for the SVF cells) with the provided infiltration cannula will ensure a better diffusion of the solution in the donor site (resulting in greater retention rates of the transferred tissue), as well as making the harvesting easier.
Cannulas size and design
The size of the harvested lobules affects the fat retention rates, as small adipose lobules ensure a better vascularisation. Adip’sculpt harvesting cannulas have been specifically designed to harvest micro lobules adapted to each indication. The blunt micro cannulas also preserve the integrity of the adipose cells, ensuring their viability.
Correlation between cannula design & tissue vascularisation
High negative pressure damages the adipose cells’ membrane. A low negative pressure (10 ml in a 60 ml syringe / 2 ml in a 10 ml syringe) will ensure optimal adipose cells viability. Adip’sculpt developed and patented the 60 ml Spinfill syringes to ensure low pressure thanks to its lockable plunger in 10 ml increments.
Correlation between oil & negative pressure
Adip’sculpt harvesting cannulas
Washing and centrifugation
Once harvested, the adipose tissue needs to be purified as it contains cell debris and toxic molecules. To increase the retention, the tissue is washed and leftover solutions removed. Adip’sculpt has shown that centrifugation is the most efficient technique; however, the parameters must be set at a low speed and short duration to concentrate the tissue while preserving cell viability.
Positive effect of washings & centrifugation with Adip’sculpt settings
Correlation between centrifugation settings & oil %
Adip’sculpt fine injection cannulas ensure the deposition of a small amount of adipose tissue per channel, maximising the vascularisation of the transferred tissue and thus ensuring high retention rates.
14G x 200 mm / 14G x 130 mm
20G x 50 mm / 17G x 100 mm
22G x 70 mm / 22G x 50 mm
Download Adip’sculpt pulications
1. New insight into lidocaine and adrenaline effects on human adipose stem cells. Girard et. al Aesthetic Plast Surg. 2013 Feb; 37 (1): 144-52 –
2. Local anesthetics : Use and Effects in autologous Fat Grafting – Girard et. al. Surgery Curr Res 2013, 3: 4 –
3. Autologous Fat Grafting in the Breast: Critical Points and Technique Improvements. Hivernaudet. al Aesthetic Plast Surg. 2015 Jun 18 –
4. Lipofilling : Critical points for successful fat grafting . Girard et. al. J Ästhet Chir 2014;7: 93-97 – Studies sponsored by Stemcis
5. Effect of washes and centrifugation on the efficacy of lipofilling with or without local anesthetic – Girard et.al. Plast Reconstr Surg Glob Open 2015; 3 (8): e496
6. Effect of centrifugation and washing on adipose graft viability : A new method to improve graft efficiency – Aesthetic Plast Surg. 2013 Feb; 37 (1): 144-52 – Hoareau et. al. J Plast Reconstr Aesthet Surg. 2013 May; 66 (5): 712-9
Studies sponsored by Stemcis
Studies & research
Comparative study of 4 protocols
Comparative studies (in the same patient) with different techniques have shown that Microfill and Macrofill have greater retention rates than other devices.
A clinical study performed on 23 patients (breast reconstruction after DIEP surgery) with the Macrofill kit have shown fat retention rates >70% (6 months post-surgery).